Figure 7: I_NaL influences the mechanical properties of LV myocytes.

(a) Quantitative data for Ca²⁺ transients in myocytes from male mice at 30–33 months, before (Tyr) and after exposure to 10 μM ranolazine (Ran) (n=19 cells from 5 hearts); data are shown as mean±s.e.m. and scatter plots. *P<0.05 versus Tyr (paired t-test). (b) Quantitative data for unloaded cell shortening properties in myocytes from male mice at 30–33 months, before (Tyr) and after exposure to 10 μM ranolazine (Ran) (n=29 cells from 4 hearts); data are shown as mean±s.e.m. and scatter plots. *P<0.05 versus Tyr (paired t-test). (c) Quantitative data for Ca²⁺ transients in myocytes from male mice at 3–6 months, before (Tyr) and after exposure to 0.5 mM 4-aminopyridine (4-AP) (n=10 cells from 2 hearts); data are shown as mean±s.e.m. and scatter plots. *P<0.01 versus Tyr (paired t-test and Wilcoxon Signed Rank Test); NP, non-parametric analysis. (d) Quantitative data for Ca²⁺ transients in myocytes from mice at 3–9 months, before (Tyr) and after exposure to 1 nM anemonia toxin-II (ATX-II) (n=14 cells from 4 hearts); data are shown as mean±s.e.m. and scatter plots. *P<0.001 versus Tyr (paired t-test and Wilcoxon Signed Rank Test); NP, non-parametric analysis.