Figure 1: FLP is a direct target of ARF7. | Nature Communications

Figure 1: FLP is a direct target of ARF7.

From: A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development

Figure 1

(a) proFLP::NLS-GFP expression in different stages of LR development. Scale bar, 50 μm. (b) qRT-PCR analysis of GATA23, ACR4, PIN3, FLP and MYB88 expression during a 12 h auxin (10 μM NAA) time course from roots of 6-day-old WT seedlings. Expression levels were normalized to those of ACTIN2. (c,d) Relative auxin-inducibility (6 h, 10 μM NAA) of (c) FLP and (d) PIN3 in WT (±10 μM DEX for 6 h), slr-1, and proARF7::ARF7-GR/arf7 arf19 (±10 μM DEX for 6 h). Samples with different letters are significantly different: P <0.05 (Fisher’s LSD mean separation test). (e) Schematic presentation of the FLP promoter (3.0 kb upstream of the translational start site at position (+1) with indication of the regions targeted for ChIP analysis (FLP_P1 and FLP_P2), and the presence of auxin response elements (AuxREs, black triangles) (f) Enrichment of the indicated DNA fragments (FLP_P1, and FLP_P2) following ChIP using anti-GR antibodies with proARF7::ARF7-GR/arf7 arf19 treated for 6 h with or without DEX. A fragment from the ACTIN2 promoter was tested as a negative control. n=3. Data are means ± s.d. ***P<0.001 (Student’s t-test).

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