Figure 2: Lateral root phenotypic analysis.

(a) Densities of the different LR stages in 6-day-old seedlings of Col-0, Ler, flp-1, flp-7, myb88, pin3-4, flp-1 myb88, flp-7 myb88 and flp-7 myb88 pin3-4. E=just emerged, not yet mature LRs; LR=mature LRs. Data shown are means ± s.d. of at least three independent experiments, each time sampling (n≥20). Samples in each stage with different letters are significantly different: P <0.05 (Fisher’s LSD mean separation test). (b) Expression pattern of the auxin response output reporter proDR5::GUS in different LR stages of WT and flp-7 myb88 (±10 μM NAA for 6 h). Scale bar, 50 μm. (c–e) Densities of (c) initiated LR primordia, (d) FCs and (e) the sum of FCs and initiated LR primordia in WT and flp-1 myb88 as visualized by proDR5::GFP. Data shown are means ± s.e.m. and are representative of at least three independent experiments. (For each experiment, n=10). **P<0.01 and ***P<0.001 (Student’s t-test).