Figure 5: In planta assessment of the relevance of ARF7 and FLP recruitment on auxin-inducibility of PIN3 and PIN3-dependent LR development. | Nature Communications

Figure 5: In planta assessment of the relevance of ARF7 and FLP recruitment on auxin-inducibility of PIN3 and PIN3-dependent LR development.

From: A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development

Figure 5

(a) Relative auxin-inducibility (10 μM NAA for 6 h) of PIN3-YFP driven by the indicated proPIN3 variants. Roots of YFP positive plants were used for qRT-PCR assay. Scheme of PIN3 promoter illustrating by colour-coding the variants of the PIN3 constructs used to complement the pin3-4 mutant. Data are means ± s.d. of at least three independent experiments. Asterisks denote Student’s t-test significance: *P<0.05, **P<0.01 and ***P<0.001. (b) Quantification of the LR densities in 8-day-old seedlings of Col-0, pin3-4, YFP-negatives segregating among transgenic plants (proPIN3_WT), proPIN3_WT, proPIN3_mA, proPIN3_mF and proPIN3_mA+mF. Box plot description: centre lines show the medians of the averages of five independent transgenic lines, calculated for at least 20 individual plants per line; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend to minimum and maximum values. n=5.

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