Figure 4: Functional analysis of the S. pombe sumoylated proteome.
From: Targeting of SUMO substrates to a Cdc48–Ufd1–Npl4 segregase and STUbL pathway in fission yeast
(a) Gene Ontology (GO)-slim ‘biological process’ analysis on the ensemble of sumoylated proteins identified in all experiments combined. The bar graph shows some of the most significantly enriched ‘process terms’ (P<0.01) in a comparison with the predicted S. pombe proteome. For comparison, the same analysis was performed on the proteins identified in crude lysates prior to SUMO enrichment. ‘Relative abundance’ refers to the fraction of proteins belonging to a given GO-category in the indicated sample (that is, entire S. pombe proteome, sumoylated proteins identified in diGly–SUMO IP or crude lysates). The analysis was performed using the GOTermMapper online tool at Princeton, and P values were calculated with a hypergeometric probability test. (b) GO enrichment analysis of ‘cellular compartment’ terms for sumoylated proteins using the AmiGo online tool. Selected enriched GO-categories (P<0.05) are arranged according to their degree of significance.
