Figure 1: Loss of GABA signalling promotes lifespan.

(a) unc-13 mutant worms are long-lived. UNC-13 is required for the release of neurotransmitters (log-rank test, P<0.001, n=74–82 for different genotypes). (b–g) Loss of octopamine (b), dopamine (c), glutamate (d), tyramine (e), serotonin (f) or acetylcholine signalling (g) modestly affect lifespan (log-rank test, P=0.755, P=0.811, P=0.371, P=0.046, P=0.069 and P=0.912, respectively. n=44–114 for different genotypes). (h) Loss of GABA signalling extends lifespan (log-rank test, P<0.001, n=57–85 for different genotypes). All lifespan assays were carried out at 20 °C and were repeated at least twice. 5-Fluoro-2′-deoxyuridine (FUDR) was included in assays involving unc-13 and unc-17 mutant worms, which are defective in egg-laying. Please see Supplementary Table 1 for detailed statistical analysis of lifespan data.