Figure 5: Weighted chemical shift perturbations (CSPs) and NMR dynamics on PmrA with and without DNA. | Nature Communications

Figure 5: Weighted chemical shift perturbations (CSPs) and NMR dynamics on PmrA with and without DNA.

From: Structure and dynamics of polymyxin-resistance-associated response regulator PmrA in complex with promoter DNA

Figure 5

CSP values for backbone amide resonances (a) and for methyl resonances (b). The black bars represent the residues that can be identified in the DNA-bound state but are missing without DNA. The dotted lines indicate the mean Δδ value plus 1 s.d. of Δδ values. (c) The R1 and R2 values of BeF3−-activated PmrA are measured in duplicate and the R2/R1 values are calculated. The average R2/R1 values are 72.5 and 40.1 for residues in REC and DBD, respectively, and are marked by dotted lines. Error bars represent fitting errors. (d) The intensity ratios of amide resonances in the DNA-bound state to free state. Error bars are noise-to-signal ratios. The mean intensity ratios are 0.46±0.10 for REC and 0.19±0.09 for DBD as indicated by the dotted lines.

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