Figure 1: Epitope mapping of five mAbs based on the combinatorial HA library displayed on the surface of yeast. | Nature Communications

Figure 1: Epitope mapping of five mAbs based on the combinatorial HA library displayed on the surface of yeast.

From: Comprehensive analysis of antibody recognition in convalescent humans from highly pathogenic avian influenza H5N1 infection

Figure 1

(a) Alignment of selected HA fragments by the five mAbs along the A/Anhui/1/2005 full-length HA glycoprotein. Each green horizontal line represents a single selected fragment. The fragment sequence between residues at positions I60 and G263 (F60–263), the minimal stretch shared and recognized by all five mAbs, is indicated by vertical dashed lines. (b) Enrichment process for F60–263 mutants that failed to bind to AVFluIgG01. The wild-type clone indicates yeast clone expressing the wild-type F60–263 and the unsorted library refers to the mutated F60–263 library before the selection and sorting process.

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