Figure 4: Characterization of antibody recognition in AH06 and SZ06 convalescent sera. | Nature Communications

Figure 4: Characterization of antibody recognition in AH06 and SZ06 convalescent sera.

From: Comprehensive analysis of antibody recognition in convalescent humans from highly pathogenic avian influenza H5N1 infection

Figure 4

(a) Alignment of selected fragments along the full-length A/Anhui/1/05 HA. The dominant regions among the selected fragments are located between the residue D53 and G263 in HA1 and G62 and Q125 in HA2, labelled by vertical dashed lines and coloured in red and blue, respectively. (b) Structural and spatial modeling of the dominant regions in HA1 (red) and HA2 (blue) on the A/Anhui/1/05 HA trimer. (c–f) Neutralizing activity of AH06 and SZ06 sera against a panel of wild-type and head/stem chimera pseudoviruses. pID/05 is short for A/Indonesia/5/2005. pCm/HK/07 is short for A/common magpie/HongKong/5052/2007. pSZ/06 is short for A/Shenzhen/406H/2006 whereas pDk/GX/04 is short for A/duck/Guangxi/1378/2004. (g–j) Neutralizing activity of AH06 and SZ06 sera against a panel of single- and multiple-mutated pseudoviruses. (k) Fold changes in ID50 of AH06 and SZ06 sera against pseudoviruses with mutations in the four vulnerable sites, compared with the parental strains. pBJ/03 is short for A/Beijing/01/03. For each wild-type and chimera pseudoviruses, neutralization sensitivity was evaluated in at least two independent experiments and the error bars indicate for the standard error of the mean (s.e.m.).

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