Figure 1: Tyrosine phosphatase SHP2 dephosphorylates wild-type and oncogenic Ras.
From: Inhibition of SHP2-mediated dephosphorylation of Ras suppresses oncogenesis
(a) HEK293 cells transfected with the indicated plasmids were treated with the indicated inhibitors, lysed, immunoprecipitated with anti-HA antibody and immunoblotted with the indicated antibodies. (b) HEK293 cells transfected with the indicated plasmids were treated with increasing concentrations of II-B08, lysed, immunoprecipitated with anti-HA antibody and immunoblotted with the indicated antibodies. (c) HEK293 cells transfected with the indicated combination of plasmids and monobodies were lysed, immunoprecipitated with anti-HA antibody and immunoblotted with the indicated antibodies. (d,e) HEK293 cells transfected with the indicated plasmids were lysed, immunoprecipitated with anti-HA antibody and immunoblotted with the indicated antibodies. (f) Bacterially purified recombinant human GST-H-Ras(WT) was subjected to in vitro kinase assay in the presence of bacterially purified recombinant human active Src kinase. Subsequent dephosphorylation reaction was conducted using increasing amounts of bacterially purified recombinant human active GST-SHP2 followed by immunoprecipitation (IP) with anti-Ras antibody and immunoblotted with the indicated antibodies. The immunoblot data are representative of at least three separate experiments. WCE, whole-cell extract.
