Figure 3: Ectopic Gr8a and Gr98b expression in bitter-sensing GRNs conferred L-canavanine sensitivity.
From: The full repertoire of Drosophila gustatory receptors for detecting an aversive compound
(a) Expression patterns of the Gr8a, the Gr66a and the Gr98b reporters as well as a cartoon indicating the location of Gr8a-, Gr66a- and Gr98b-expressing sensilla in the labellum. The scale bar represents 50 μm. (b) Ectopic expression of Gr8a and Gr98b in bitter-sensing GRNs (I-type sensilla) conferred L-canavanine sensitivity to sensilla that did not normally respond to L-canavanine. A schematic representation of a sensilla depicting the ectopic expression experiment (above) and representative traces (below) evoked by 30 mM L-canavanine from I4 sensilla of control (UAS-Gr8a,UAS-Gr98b) and UAS–Gr8a,UAS–Gr98b;Gr33a–GAL4 flies. (c) Response frequencies evoked by 30 mM L-canavanine after ectopic expression of Gr8a and Gr98b in the indicated sensilla. UAS–Gr8a,UAS–Gr98b flies were the negative controls. n=4–14. **P<0.01 (Mann–Whitney U-test). Medians and quartiles are shown.
