Figure 1: Mitosis in polarized MDCK cells. | Nature Communications

Figure 1: Mitosis in polarized MDCK cells.

From: Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement

Figure 1

(a) (i) MDCK cells expressing actin-mCherry (red) and eGFP-labelled histones (H2B-eGFP, green) and (ii) MDCK cells expressing E-cadherin-eGFP (green) and nuclei stained with Hoechst 33342 (grey). Cells were plated on permeable filter supports and grown for 3 days before data acquisition (see Supplementary Fig. 1 for MDCK cells grown for 14 days). The eGFP-labelled histones allowed us to determine the mitotic phases of MDCK cells (see Methods). (i) Confocal microscopy images of polarized MDCK cells expressing actin-mCherry and H2B-eGFP showing mitotic cells rounded up in metaphase (yellow arrow). (ii) Confocal microscopy image of MDCK cells expressing E-cadherin-eGFP that show extensive contacts between metaphase (yellow arrow) and interphase cells. (b) Schematic drawing of cell division in the epithelial layer. Scale bars, 10 μm.

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