Figure 5: Viability and apoptosis of MDCK cells confined by micropillars. | Nature Communications

Figure 5: Viability and apoptosis of MDCK cells confined by micropillars.

From: Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement

Figure 5

(a) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay of MDCK cells cultured on micropillar arrays for 3 h in the presence of different chemical compounds that perturb cellular processes. For each condition, mean and s.d. are given. (b) Percentage of apoptotic (red) and living (green) cells on the flat surface in the presence of different perturbants (ncells=120, 140, 80, 95, 100, 113, 105, 95, 80). (c) Percentage of apoptotic and living cells on micropillar arrays (ncells=200, 150, 180, 210, 220, 150, 170, 210, 170). Apoptotic and living cells were quantified using CellProfiler (see Methods). Micropillar arrays used had average interpillar distances of ≈6.8 μm. Mann–Whitney P values indicate the significance relative to the control (untreated) cells. *P<0.01, **P<0.001. Perturbants are described in Supplementary Table 1.

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