Figure 4: Glutaminase inhibition limits HSV-1 and influenza A replication.

(a) Glutamine consumption rates of primary human foreskin fibroblasts (HFF) mock infected or infected with HSV-1 (herpes simplex virus 1) at an MOI of 1 for 24 h in the presence of DMSO or 1.25 μM CB-839. (b) Infectious viral titer (measured by plaque assay) of supernatant from HFF cells infected with HSV-1 at an MOI of 0.01 for 72 h in the presence of DMSO, 1.25 μM CB839, or in the absence of exogenous glutamine (−Q). (c) Glutamine consumption rates of NHBE cells mock infected or infected with influenza A virus at an MOI of 1 for 24 h in the presence of DMSO or 1.0 μM CB-839. (d) Infectious viral titer (measured using a TCID₅₀ end point dilution assay) of the supernatant from NHBE cells infected with influenza A at an MOI of 1 for 24 h in the presence of DMSO or 1.0 μM CB-839. For (a–d), error bars denote s.d. (n=3), *P<0.05; **P<0.01. Student’s t-test.