Figure 1: NMR and ITC analysis of the cpSRP43–A3CT interaction.
From: Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction
(a) Scheme of the cpSRP system in post-translational LHCP targeting. (b) Schematic representation of cpSRP43 and A3CT constructs used in this study. (c) NMR chemical shift perturbations (CSPs) of CD2CD3 on titration with A3CT. Major CSPs are observed only for residues located in CD3. (d) Characterization of the interaction between cpSRP43 and A3CT. ITC titrations are shown for cpSRP43 with A3CT (left) and cpSRP43 with A3CT IVp (middle). KD values for relevant ITC measurements are listed in a table (right). (e) CSPs of CD2CD3 on titration with RRKRp. Major spectral changes on RRKRp binding occur only for residues located in CD2. (f) CSPs observed in A3CT on titration with unlabelled CD2CD3. Significant CSPs are observed for residues located at the C terminus of A3CT (motif IV and flanking residues). CSPs with a negative value indicate residues that could not be assigned in the bound state.
