Figure 2: TRPC6 inhibited γ-secretase cleavage of APP (C99), but not that of other substrates. | Nature Communications

Figure 2: TRPC6 inhibited γ-secretase cleavage of APP (C99), but not that of other substrates.

From: TRPC6 specifically interacts with APP to inhibit its cleavage by γ-secretase and reduce Aβ production

Figure 2

(a) Aβ levels in the medium of COS7C99 stable cells transfected with YFP or TRPC6 for 2 days (n=3–4). (b) Immunoblot analysis of AICD in HEK293C99 cells transfected with YFP or TRPC6 for 2 days. (c) Quantification of the ratio of AICD/C99 (n=3). (d) Immunoblots of presenilin 1 (PS1), PS2, nicastrin (Nct) and presenilin enhance 2 (Pen2) in HEK293APP cells transfected with TRPC6 for 2 days. (e) γ-Secretase activity in the in vitro fluorogenic substrate assay of HEK293APP cells transfected with TRPC6 for 2 days (n=3–5). (f) Immunoblot analysis of Notch intracellular domain (NICD) in HEK293APP cells transfected with NotchΔE-myc for 1 day and then further transfected with YFP or TRPC6 for 2 days. (g) Quantification of the ratio of NICD/NotchΔE (n=4). (h) Immunoblots of CTF1 and CTF2 of E-/N-Cadherin in HEK293APP cells transfected with YFP or TRPC6 for 2 days. (i) Quantification of CTF2/CTF1 of E-/N-Cadherin (n=3–4). CTRL, transfection with YFP or pcDNA3.1. L658,458: γ-secretase inhibitor. Data were presented as means±s.e.m. of indicated numbers of independent experiments. Two-tailed Student’s t-test was performed. *P<0.05, **P<0.01 versus CTRL.

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