Figure 3: Self-renewal and differentiation in aneuploid brains.

(a) Pictures of WT, SakOE,mad2 and bub3 brains stained for Dpn (middle and in purple to label Nbs) and Prospero (Pros) (right, in green to label GMCs). DNA is shown in blue. The yellow dashed line marks the CB region. Scale bar, 50 μm. (b) Dot plot chart showing the quantification of the ratio between nuclear Pros⁺ (GMCs) and Dpn⁺ (Nbs) in WT (red dots, n=19 lobes), SakOE,mad2 (green dots, n=15 lobes) and bub3 (blue dots, n=11 lobes) brains. Each dot represents the ratio from one brain lobe. The line represents the mean and the error bars the s.d. The raw data of quantifications are given in Supplementary Table 1. Statistical significance (SS) was assessed by an unpaired t-test. **(P<0.01). (c) Dot plot chart showing the quantification of the ratio between Elav⁺ (neurons) per CB area in WT (red dots, n=20 lobes), SakOE,mad2 (green dots, n=26 lobes) and bub3 (blue dots, n=16 lobes) brain lobes. Each dot represents the ratio from one brain lobe. The line represents the mean and the error bars the s.d. The corresponding pictures and the raw data of quantification are shown in Supplementary Fig. 7 and Supplementary Table 2. Statistical significances were assessed by an unpaired t-test. **(P<0.01). (d) Pictures of WT (top) and bub3^RNAi (middle and bottom) clones expressing mCD8 (shown in green to label the clone) and stained for Dpn (labelling Nbs, middle and in purple in the merge panel) and for Pros (labelling GMCs, right panel and in red in the merge channel). The bottom picture shows a clone that comprises a large cell not expressing Dpn and several GMCs. Scale bar, 5 μm. (e) Dot plot chart showing the quantification of clone area in WT (red dots) and bub3^RNAi (blue dots) clones. Each dot represents a clone. The line represents the mean and the error bars the s.d. Statistical significance was assessed by an unpaired t-test. **(P=0.0078). (f) Dot plot chart showing the quantification of number of cells per clone in WT (red dots) and bub3^RNAi(blue dots) clones. Each dot represents a clone. The line represents the mean and the error bars the s.d. Statistical significance was assessed by unpaired t-test. ***(P=0.0006).