Figure 7: Aneuploid brains have reduced tumorigenic capacity.
From: Aneuploidy causes premature differentiation of neural and intestinal stem cells
(a). Pictures of WT adult host flies transplanted with Tubulin-GFP (left) or Tubulin-GFP; SakOE,mad2 (right) pieces of L3 brains. Scale bars, 400 μm. (b) Graph bars showing the quantification of tumour formation for indicated genotypes. Numbers on top of each column indicate the number of transplants performed. Statistical significance was determined using Fet *(P=0.03). (c) Immunostaining of WT, SakOE,mad2 and bub3 L3 Nbs with α-tubulin (left and in red in the merged panel), dPLP (Drosophila pericentrin-like protein) and aPKC (middle panel, shown in green in the merged panel) antibodies. DNA is shown in blue. Scale bar, 2 μm. (d) Quantification of mitotic spindle orientation at anaphase in WT (n=24), SakOE, mad2 (n=45) and bub3 (n=24). Only SakOE,mad2 Nbs present spindle position defects. Statistical significance was determined using unpaired t-test ****(P<0.0001).
