Figure 1: Rv1988 interacts with histone H3. | Nature Communications

Figure 1: Rv1988 interacts with histone H3.

From: Mycobacteria modulate host epigenetic machinery by Rv1988 methylation of a non-tail arginine of histone H3

Figure 1

(a) Lysate of HEK293 cells, transfected with SFB-Rv1988 or pcDNA3.1 (control) constructs and pulled down with streptavidin beads was probed with antibodies as indicated. In all, 5% of the whole-cell lysate was used as Input. Streptavidin pulls down SFB-Rv1988. PD: pull down. (b) Immunoprecipitation was performed with H3 antibody on SFB-Rv1988-transfected HEK293 cells and probed with antibodies as indicated. IP was also done with IgG as a control. In all, 10% of the whole-cell lysate was used as Input. Anti-FLAG antibody detected SFB-Rv1988.

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