Figure 3: Rv1988 is secreted by mycobacteria. | Nature Communications

Figure 3: Rv1988 is secreted by mycobacteria.

From: Mycobacteria modulate host epigenetic machinery by Rv1988 methylation of a non-tail arginine of histone H3

Figure 3

Rv1988 secretion in M. bovis BCG (a) and Rv1988-transformed M. smegmatis (b). Western blot showing cell lysate and culture filtrate of M. bovis BCG and Rv1988::M. smegmatis was probed with Rv1988 and GroEL1 antibodies. Mb2010, Rv1988 homologue in M. bovis BCG (a) or Rv1988 (b) was detected by Rv1988 antibody (upper panel) in culture filtrate (lanes 1, 3) and cell lysate (lanes 2, 4) of two independent cultures. (c) Mutation in the Tat-signal sequence abolishes the secretory potential of Rv1988. Culture filtrate and cell lysate for Rv1988-6XHis and Rv1988R8A/R9A-6XHis-transformed M. smegmatis was probed for the presence of Rv1988. 1: cell lysate and 2: culture filtrate for Rv1988-6XHis::M. smegmatis; 3: cell lysate and 4: culture filtrate for Rv1988R8A/R9A-6XHis::M. smegmatis. GroEL1 (a non-secretory protein) antibody was used to examine cell lysis (lower panel).

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