Figure 4: Rv1988 localizes to the host chromatin upon infection. | Nature Communications

Figure 4: Rv1988 localizes to the host chromatin upon infection.

From: Mycobacteria modulate host epigenetic machinery by Rv1988 methylation of a non-tail arginine of histone H3

Figure 4

(a) Mouse peritoneal macrophages infected either with GFP or Rv1988-GFP expressing M. smegmatis analysed 48 h after infection by confocal microscopy. Scale bar, ∼10 μm. Inset shows a ‘zoomed in’ view of the region marked by red box. Scale bar, ∼1 and 2 μm (upper and lower panel, respectively). Some of the bacilli are outlined in red. Note rod-shaped structures stained by GFP in GFP::M. smegmatis-infected peritoneal macrophages as against a diffused GFP signal in Rv1988-GFP::M. smegmatis-infected peritoneal macrophages. (b) Rv1988 localizes to the host chromatin. Subcellular fractions (as indicated below the panels) of THP1 cells infected with Rv1988-6XHis::M. smegmatis (lanes 1, 4, 7), Rv1988R8A/R9A-6XHis::M. smegmatis (lanes 2, 5, 8) or pVV16::M. smegmatis (lanes 3, 6, 9) were probed with the indicated antibodies. Histone H3 and GAPDH antibodies were used as control for chromatin and cytoplasmic fractions, respectively. GroEL1 antibody was used to show that mycobacterial cells were not lysed.

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