Figure 5: Rv1988 mediates transcriptional repression. | Nature Communications

Figure 5: Rv1988 mediates transcriptional repression.

From: Mycobacteria modulate host epigenetic machinery by Rv1988 methylation of a non-tail arginine of histone H3

Figure 5

(a) Line diagram describing luciferase reporter gene assay strategy in HEK293 cells. The 5 UAS sites for GAL4 binding in pG5luc are shown as open rectangles. The filled shapes indicate fusion protein produced by pBIND-Rv1988 vector construct. Raised arrow indicates transcription start site. (b) Rv1988 represses transcription. Luciferase activity from pG5luc measured 48 h after co-transfection of various combinations of constructs as indicated. The values are represented as ratio w.r.t. luciferase activity of pG5luc vector alone and were normalized for transfection efficiency w.r.t. GFP expression from a co-transfected GFP construct. (c) ChIP analysis for association of various histone modifications (indicated below the x-axis) with the reporter luciferase gene promoter. Enrichment for each modification is represented as percentage input. The error bars represent s.d. Student’s t-test, *P<0.05; ***P<0.0001. (d) Enrichment of H3-arginine dimethylation. ChIP performed on HEK293 cells transfected with Rv1988 or vector alone constructs using histone H3 antibody and analysed for arginine dimethylation (upper panel) and histone H3 (lower panel).

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