Figure 5: MiR-193b targets c-KIT expression and thereby modulates signalling in HSPCs. | Nature Communications

Figure 5: MiR-193b targets c-KIT expression and thereby modulates signalling in HSPCs.

From: STAT5-regulated microRNA-193b controls haematopoietic stem and progenitor cell expansion by modulating cytokine receptor signalling

Figure 5

(a) PamGene array volcano plots of tyrosine and serine/threonine kinases in BM cells derived from miR-193b−/− and miR-193b+/+ mice. Altered peptides and potential kinases (in brackets) are shown. N=4 independent experiments. (b) Target scan analysis of the 3′-untranslated region of murine c-Kit, including the location and conservation of the miR-193b-binding site. Sequence differences between the species are highlighted in red. (c) c-Kit mRNA expression upon miR-193b ectopic expression in LT-HSCs via qPCR. C-Kit mRNA expression was normalized to Gpdh mRNA. Transduction efficiencies were 94% and 90% for control and miR-193b, respectively. N=3 independent experiments. (d) FACS analysis of c-KIT surface expression upon miR-193b ectopic expression in LT-HSCs via FACS. A representative FACS plot and relative quantification of the c-KIT expression normalized to the CTRL (control) transduced cells are displayed. N=3 independent experiments. (e) Competitive transplantation of LT-HSCs that were lentivirally transduced with either miR-193b or CTRL 24 h prior transplantation. The transduction efficiency was 26% in both groups. Donor cell engraftment was measured in the peripheral blood after 4 weeks. N=5–6 mice per group. Mann–Whitney test. All data are represented as the mean±s.d. ***P<0.001.

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