Figure 6: Ectopic c-Kit expression can rescue miR-193b-mediated effects in HSPCs. | Nature Communications

Figure 6: Ectopic c-Kit expression can rescue miR-193b-mediated effects in HSPCs.

From: STAT5-regulated microRNA-193b controls haematopoietic stem and progenitor cell expansion by modulating cytokine receptor signalling

Figure 6

(a) Endogenous c-KIT protein expression in BM cells of miR-193b−/− and miR-193b+/+ mice measured via FACS. N=4 mice per group. (b) Rescue of HSPC survival by ectopic c-KIT expression. Re-expression of c-KIT lacking the 3′-untranslated region in miR-193b-transduced LT-HSCs via lentiviral co-infection and culture for several days. (c) Representative FACS plots and quantification of cultured cells at day 9. Ratio of double transduced cells (day 9/day 3) displayed as the mean±s.d. N=3 independent experiments. All data are represented as the mean±s.d. MFI, mean fluorescence intensity. *P<0.05; NS, not significant.

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