Figure 1: Clustered distribution of the β3-subunit.
From: Disinhibition of olfactory bulb granule cells accelerates odour discrimination in mice
(a) Immunostaining using a β3-subunit-selective antibody (red hot colour palette) in horizontal OB slices infected with rAAV-mGFP (grey). The images represent single confocal frames. From left to right, GCL, MC layer (MCL), EPL and glomerular layer (GL). Scale bar, 200 μm. (b) Abundance of β3-subunit immunosignal in the EPL and GL relative to the GCL. Representative image used to quantify the grey-scale intensity values (here using the rainbow colour palette). See Methods for further details. Ratios relative to GCL in EPL (1.33±0.04; n=16; ***P<0.001), GL (0.82±0.04; n=16; **P<0.01; Bonferroni’s multiple comparison test). The mean ratios±s.e.m. Scale bar, 58 μm. (c) Distribution of β3-subunit clusters (red hot colour palette) within a single GC (grey). Images represent maximum-intensity projections of a confocal stack extending over 70 image frames, after frame-by-frame multiplication as illustrated in Supplementary Fig. 1C–F. Grey-scale levels adjusted for display. Scale bar, 0.25 μm. (d–g) 3D reconstructions of regions shown in c. 3D reconstruction procedure is illustrated in Supplementary Figs 2 and 3. The cartesian axes x (blue), y (red) and z (white) show the rotation of the reconstructed region from origin. The width of the image corresponds to ∼12.5 μm in d–f and 25 μm in g. Panels: mGFP, membrane-labelled GC; GABRB3, all immunosignals in the respective volume; mGFPxGABRB3, immunosignals residing inside the GC; merge, d,e show all GABRB3 immunosignals surrounding the GC; f,g show GABRB3 immunosignals residing within the GC. (h) Distribution of β3-subunit clusters (red hot colour palette) within the distal dendritic tree (grey). The images represent maximum-intensity projections of a confocal stack extending over 124 image frames. Grey-scale levels adjusted for display. Scale bar, 10 μm.
