Figure 3: Binding of UBA immobilizes the αC-out kinase conformation.
From: Structural insight into the mechanism of synergistic autoinhibition of SAD kinases
(a) Comparison of the autoinhibited SAD-A with the active conformations of PKA (PDB code: 1ATP) and Snf1 (2FH9). For clarity, only strands β3, helices αC and activation segments of PKA (marine blue) and Snf1 (green) are displayed. The conserved Lys and Glu side chains and two hydrophobic residues in the activation segment of SAD-A are highlighted as sticks. (b) Effects of the UBA mutations on the catalytic efficiencies of KD-UBA. The kcat/Km values are determined as that in Supplementary Table 1.
