Figure 6: C. elegans SAD-1 mutations mislocate presynaptic vesicle clusters to dendrite of DA9 neuron.
From: Structural insight into the mechanism of synergistic autoinhibition of SAD kinases
(a) Sequence alignment of UBA-α3 and AIS from mouse SAD-A and C. elegans SAD-1. Residues involved in intramolecular interactions are shown in bold, and those mutated in following assays are highlighted in blue or green. (b) Localization of synaptic vesicle-associated GFP::RAB-3 in wild-type animals. (c–g) Distribution of GFP::RAB-3 in animals expressing single-copy insertion of wild-type and mutant sad-1. Scale bar, 10 μm. (h) Quantification of ectopic dendritic puncta in wild-type and mutant animals. The columns represent the percentage of animals with 0, 1–3, 4–6, or >6 extra presynaptic puncta in the dendrite.
