Figure 1: NO signalling is upregulated in GFAPR79H transgenic flies.
From: Nitric oxide mediates glial-induced neurodegeneration in Alexander disease
(a) Western blot shows increased expression of Nos in GFAPR79H transgenic flies. The blot was reprobed for actin to illustrate equivalent loading. Quantification of Nos protein levels in control and GFAPR79H transgenic flies is shown in the graph at the right. *P=0.0078, t=6.371, df=3; unpaired t-test, n=4. (b) NADPH-diaphorase staining is increased in the brain of GFAPR79H transgenic flies. Quantification is shown in the graph at the right. *P=0.0021, t=5.800, df=5; unpaired t-test, n=6 per genotype. (c) Time-lapse imaging reveals increased nitric oxide production in vivo in GFAPR79H transgenic flies with a nitric oxide sensor Cu2(FL2E). Scale bar, 10 μm. *P<0.05, t=2.833 (6 min); 3.173 (8 min); 3.137 (10 min); two-way ANOVA, n=7 per genotype. (d) Nitrotyrosine (N-tyr)-modified proteins are increased in GFAPR79H flies. *P=0.0273, t=2.902, df=6; unpaired t-test, n=4 per genotype. (e) Significantly increased cGMP in GFAPR79H transgenic flies as assayed by the chemiluminescent immunoassay kit. **P<0.0001, t=6.109, df=16; unpaired t-test, n=9 per genotype. Flies were 20 days old in all panels. Control (Ctrl) is repo-GAL4/+. GFAPR79H is repo-GAL4, UAS-GFAPR79H/+.
