Figure 4: Less macrophages in plaques from M4K4 ECKO and KD mice.
From: Endothelial protein kinase MAP4K4 promotes vascular inflammation and atherosclerosis
(a,b) Messenger RNA was prepared from whole aortas, and qPCR was performed. (a) Macrophage markers F4/80 and Cd68. (b) Chemokines Ccl2, Cxcl1, Ccl3, Ccl4, Ccl5, Ccl7, Cxcl9 and Cxcl10. Data represent the mean±s.e.m. as normalized to 36b4 (*P<0.05, **P<0.005, N=9–10). (c,d) Homing of GFP leukocytes into atherosclerotic lesions 48 h after intravenous injection into control or MAP4K4 KD mice that were fed WD for 16 weeks. (c) Fluorescence micrograph of atherosclerotic plaque demonstrating four GFP leukocytes within the aortic arch. The dashed line indicates the plaque border. Inset, magnification of three GFP leukocytes. Left, 4,6-diamidino-2-phenylindole; middle, GFP; right, merge. Scale bars, 100 μm. (d) Quantification of GFP leukocytes per square millimetre of plaque. Data represent the mean±s.e.m. (**P<0.005, N=4,7).
