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Dissociation of Lipid Components and Reconstitution at – 75° C of Mg2+ Dependent, Na+ and K+ Stimulated, Adenosine Triphosphatase in Rat Brain

Nature New Biology volume 230, pages 148–150 (1971)Cite this article

Abstract

MEMBRANE enzymes, because of their lipid content, are insoluble in water and usually solubilized as micelles in aqueous solution by detergents for biochemical study. Direct study of lipid components by means of organic liquids in which they dissolve leads at once to the denaturation of the enzyme. At temperatures appreciably colder than 0° C, however, organic solvents may leave enzymatic activity intact1–3, making it possible to study enzyme reactions.

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Author notes

  1. TETSUYA NOGUCHI: On leave of absence from Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.

Authors and Affiliations

  1. Departments of Biochemistry and Neurology, New York Medical College, New York, New York, 10029

    TETSUYA NOGUCHI & SIMON FREED

  2. Chemistry Department, Brookhaven National Laboratory, Upton, New York, 11973

    TETSUYA NOGUCHI & SIMON FREED

Authors
  1. TETSUYA NOGUCHI
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  2. SIMON FREED
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NOGUCHI, T., FREED, S. Dissociation of Lipid Components and Reconstitution at – 75° C of Mg2+ Dependent, Na+ and K+ Stimulated, Adenosine Triphosphatase in Rat Brain. Nature New Biology 230, 148–150 (1971). https://doi.org/10.1038/newbio230148a0

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