Abstract
PROTEIN aggregates are often dissociated by sodium dodecyl sulphate (SDS) and the subunit molecular weight then estimated by gel-electrophoresis1–3. This method uses internal standards and at the lower range of molecular weights (approximately 10,000) it may be difficult to find well defined standards. For these proteins it is necessary to utilize the analytical ultracentrifuge.
This is a preview of subscription content, access via your institution
Access options
References
Reynolds, J. A., and Tanford, C., J. Biol. Chem., 245, 5161 (1970).
Weber, K., and Osborne, M., J. Biol. Chem., 244, 4406 (1969).
Dunker, A. K., and Rueckert, R. R., J. Biol. Chem., 244, 5074 (1969).
Hersh, R. T., and Schachman, H. K., Virology, 6, 234 (1958).
Carusi, E. A., and Sinsheimer, R. L., J. Mol. Biol., 7, 388 (1963).
Spragg, S. P., Anal. Chim. Acta, 38, 137 (1967).
Goodman, R. F., and Spragg, S. P., Ann. NY Acad. Sci., 164, 294 (1969).
Theorell, H., Biochem., 268, 46 (1934).
Antonini, E., Phys. Rev., 45, 1 (1965).
Knippers, R., and Hoffman-Berling, H., J. Mol. Biol., 21, 281 (1966).
DeGroot, K., Hoenders, H. J., Gerding, J. J. T., and Bloemetidal, H., Biochim. Biophys. Acta, 207, 202 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BARNETT, W., SPRAGG, S. Estimation of Molecular Weights of Protein-SDS Complexes. Nature New Biology 234, 191–192 (1971). https://doi.org/10.1038/newbio234191a0
Received:
Revised:
Issue date:
DOI: https://doi.org/10.1038/newbio234191a0