Abstract
SPIN labelling has often been used to study conformational transitions in proteins1. Electron spin resonance (ESR) spectra of the iminoxyle radical attached to certain sites of a protein molecule indicate the spatial rearrangements of adjacent molecular groups1. We have synthesized a new spin label, which contains a isocyanide group and therefore specifically binds to iron-porphyrin moieties of haemoproteins. The iminoxyle radical with—N=C group was prepared by reaction of chloroform with 2,2,6,6,-tetramethyl-4-aminopiperidyl-N-oxyle in the presence of potassium hydroxide.
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REICHMAN, L., ANNAEV, B., BELOVA, V. et al. Iminoxyle Radical with Isocyanide as a Spin Label for Haemoproteins. Nature New Biology 237, 31–32 (1972). https://doi.org/10.1038/newbio237031b0
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DOI: https://doi.org/10.1038/newbio237031b0
