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Isolation of Protoplasts from Pollen Tetrads

Abstract

PRODUCTION of haploid plants by anther culture is restricted to only a few taxa1. If protoplasts could be isolated from pollen tetrads they might behave in culture similarly to somatic cell protoplasts2, and serve as the starting material for the production of haploid plants for a wide range of plant species. Such isolated microspore protoplasts might also be suitable for fusion studies in relation to somatic hybridization of plants2.

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References

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BHOJWANI, S., COCKING, E. Isolation of Protoplasts from Pollen Tetrads. Nature New Biology 239, 29–30 (1972). https://doi.org/10.1038/newbio239029a0

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  • DOI: https://doi.org/10.1038/newbio239029a0

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