Abstract
TREATMENT of Escherichia coli cells with lysozyme and EDTA partially removes the outer layer of the cell wall containing lipopolysaccharide (LPS), leaving osmotically unstable spheroplasts1. These can be infected with phage nucleic acid2 and can produce viable phage particles. Removal of LPS-containing phage receptor sites3–5, however, leaves spheroplasts resistant to infection by intact phages1. We now show that LPS, obtained from phage-sensitive cells by aqueous phenol extraction, can provide functional phage receptor sites to spheroplasts prepared from cells lacking receptor sites.
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References
Birdsell, D., & Cota-Robles, E., J. Bacteriol., 93, 427 (1967).
Sinsheimer, R. L., in Methods in Enzymology (edit. by Grossman, L., and Moldau, K.), 12, part B, 850 (Academic Press, New York, 1968).
Weidel, W., Kock, G., & Lohss, F., Z. Naturf., 9b, 398 (1954).
Kalckar, H., Laursen, P., & Rapin, A., Proc. US Nat. Acad. Sci., 56, 1852 (1966).
Wilson, J., Luftig, R., & Wood, W., J. Mol. Biol., 51, 423 (1970).
Watson, G., & Paigen, K., J. Virol. (in the press).
Osborn, M. J., in Methods in Enzymology (edit. by Neufeld, E. F., and Ginsburg, V.), 8, 161 (Academic Press, New York, 1966).
Rapin, A., Kalcar, H., & Alberico, L., Arch. Biochem. Biophys., 128, 95 (1968).
Guthrie, G., & Sinsheimer, L., Biochim. Biophys. Acta, 72, 290 (1963).
Anderson, E. H., Proc. US Nat. Acad. Sci., 32, 120 (1946).
Strauss, J., J. Mol. Biol., 10, 422 (1964).
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WATSON, G., PAIGEN, K. Intercellular Transfer by Phage Receptor Site Lipopolysaccharide. Nature New Biology 239, 120–122 (1972). https://doi.org/10.1038/newbio239120a0
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DOI: https://doi.org/10.1038/newbio239120a0
