Abstract
Serum, used to supplement cell culture media, is usually sterilized by filtration because heat-treatment may adversely affect its growth-promoting properties. This filter-sterilization procedure is, however, unreliable because viruses and mycoplasmas may easily pass through the filter. As another approach, we have studied the question of whether serum sterilized by peracetic acid can be used in cell and tissue cultivation. Preliminary results1,2 indicated that this was possible, and we have attempted to establish the best method of peracetic acid treatment and to show that serum treated in this way is suitable for cell cultivation.
This is a preview of subscription content, access via your institution
Access options
References
Sprössig, M., Mücke, H., and Wutzler, P., Z. Ges. Hyg., 17, 620 (1971).
Sprössig, M., 13, Meeting of the Central Sterilising Club, Edinburgh (1971).
Mücke, H., and Sprössig, M., Pharmazie, 22, 444 (1967).
Schweizer, H., Zentbl. Bakt. Parastkde I Orig., 206, 175 (1968).
Schweizer, H., Z. Med. Labortechnik, 12, 328 (1971).
Sprössig, M., Mücke, H., and Tilgner-Peter, Ch., Pharmazie, 22, 517 (1967).
Koch, H. A., Sprössig, M., and Mücke, H., Pharmazie, 22, 520 (1967).
Sprössig, M., and Mücke, H., Pharmazie, 23, 665 (1968).
Sprössig, M., Gesundh Wes. Desifgekto., 25, 1045 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SCHWEIZER, H., SPRÖSSIG, M., MÜCKE, H. et al. Peracetic Acid as Sterilizing Agent for Culture Serum. Nature New Biology 240, 61–62 (1972). https://doi.org/10.1038/newbio240061a0
Received:
Issue date:
DOI: https://doi.org/10.1038/newbio240061a0
