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Banding of Human Chromosomes by Protein Denaturation

Nature New Biology volume 241, pages 142–143 (1973)Cite this article

Abstract

METHODS now used for chromosome identification fall into three major categories: (1) the use of quinacrine fluorochromes1–4; (2) alkali-heating techniques, which give similar patterns but with minor variations5–7; and (3) use of anyone of several proteolytic enzymes8–11. We report a fourth group of protein-denaturing substances that effectively band human chromosomes: urea, 5 M; two strongly anionic detergents (sodium lauryl sulphate, 1.25%; sodium deoxycholate, 1.25%); ‘Nonidet P40’, 5% (Shell Canada Ltd), a nonionic detergent; and ‘7X’, 1 % (Linbro Chemical Co.Inc, New Haven, Conn.), a commercial detergent for cleaning laboratory glassware.

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Authors and Affiliations

  1. Atlantic Research Centre for Mental Retardation, and Departments of Paediatrics and Microbiology, Dalhousie University, Halifax, Nova Scotia

    C. L. Y. LEE, J. P. WELCH & S. H. S. LEE

Authors
  1. C. L. Y. LEE
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  2. J. P. WELCH
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  3. S. H. S. LEE
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LEE, C., WELCH, J. & LEE, S. Banding of Human Chromosomes by Protein Denaturation. Nature New Biology 241, 142–143 (1973). https://doi.org/10.1038/newbio241142a0

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