Abstract
THE physiological role of the enzyme polynucleotide Phosphorylase (PNPase), which seems to exist in many different bacteria (unpublished work), is not known. This enzyme can polymerize or depolymerize single stranded polyribonucleotides in a truly reversible reaction (ApUpGpCp)n+nPi⇄nADP +nUDP+nGDP+nCDP1−3. The phosphorolysis of polyribonucleotides by this enzyme proceeds processively from the 3′ end to the 5′ end of the polynucleotide chain4.
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References
Grunberg-Manago, M., Ortiz, P. J., and Ochoa, S., Biochim. Biophys. Acta, 20, 269 (1956).
Singer, M. F., in Progress in Nucleic Acid Research (edit, by Cantoni, G. L., and Davies, D. R.), 245 (Harper and Row, New York, 1962).
Kimhi, Y., and Littauer, U. Z., J. Biol. Chem., 243, 231 (1968).
Klee, C. B., and Singer, M. F., J. Biol. Chem., 243, 923 (1968).
Reiner, A. M., J. Bact., 97, 1431 (1968).
Reiner, A. M., J. Bact., 97, 1437 (1968).
Krishna, R. V., and Apirion, D., J. Bact. (in the press).
Chaney, S. G., and Boyer, P. D., J. Mol. Biol., 64, 581 (1972).
Simon, M., and Apirion, D., Genetics, 71, 1 (1972).
Nossal, N. G., and Singer, M. F., J. Biol. Chem., 243, 913 (1968).
Pato, M. L., and Von Meyerburg, K., Cold Spring Harbor Symp. Quant. Biol., 35, 497 (1970).
Kennell, D., and Magasanik, B., Biochim. Biophys. Acta, 81, 418 (1964).
Kepes, A., Biochim. Biophys. Acta, 76, 293 (1963).
McFall, E., Genetics, 55, 91 (1967).
Chou, J. F., and Singer, M. F., J. Biol. Chem., 245, 1005 (1970).
Thang, M. N., Thang, D. C., and Grunberg-Manago, M., Biochem. Biophys. Res. Commun., 28, 374 (1967).
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KRISHNA, R., ROSEN, L. & APIRION, D. Increased Inactivation and Degradation of Messenger RNA in an Escherichia coli Strain containing a Thermolabile Polynucleotide Phosphorylase. Nature New Biology 242, 18–20 (1973). https://doi.org/10.1038/newbio242018a0
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DOI: https://doi.org/10.1038/newbio242018a0
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