Abstract
Rough membranes reconstituted by combination in vitro of hepatic polysomes and rough microsomes stripped of their-endogenous polysomes will incorporate amino-acids and vectorially transport the puromycin-released protein across the membrane. Both of these rough membrane activities were quantitatively similar to rough microsomes; however, the membrane binding sites appeared undiscriminatory in binding nearly all postmitochondrial polysomes with equal affinity.
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SHIRES, T., EKREN, T., NARURKAR, L. et al. Protein Synthesis on Rat Liver Polysome-Membrane Complexes formed in vitro and Disposition of the Discharged Chains. Nature New Biology 242, 198–201 (1973). https://doi.org/10.1038/newbio242198a0
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DOI: https://doi.org/10.1038/newbio242198a0
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