Abstract
Restriction endonuclease EcoRII makes about twenty double-stranded breaks per molecule of λh80 DNA. The 5′-terminal sequences are pC-C-A-G-G and pC-C-T-G-G. These are complementary and rotationally symmetrical, showing how the enzyme may produce DNA fragments with short cohesive ends.
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BIGGER, C., MURRAY, K. & MURRAY, N. Recognition Sequence of a Restriction Enzyme. Nature New Biology 244, 7–10 (1973). https://doi.org/10.1038/newbio244007a0
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DOI: https://doi.org/10.1038/newbio244007a0
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