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Measurement of RD114 Virus Nucleotide Sequences in Feline Cellular DNA

Nature New Biology volume 244, pages 62–64 (1973)Cite this article

Abstract

MCALLISTER et al.1 recently reported the observation of a type-C virus released from cultured human rhabdomyosarcoma cells following passage in foetal kittens. The parent cell line (RD cells) contained no virus before passage. However, a cell line, RD114, established from a brain tumour in an inoculated kitten released particles with morphological and biological properties of a mammalian RNA tumour virus. The obvious explanation that the appearance of RD114 virus (RDV) represented an infection of the human RD cells by an endogenous feline oncornavirus was undermined by the observation of striking antigenic and biological differences between RDV and known feline viruses. Briefly listed, these differences included the observation that: (1) RDV grows well on human and primate cells but not on feline cells1; (2) the group-specific antigen of known feline type-C viruses was absent from RDV as were feline envelope antigens as judged by interference and viral neutralization tests; further, antigens of other known mammalian type-C viruses were not detected, with the exception of the interspecific antigen (gs-3)1,2; (3) the constituent RNA-dependent DNA polymerase of RDV did not react with antisera against feline leukaemia virus (FLV) DNA polymerase and other mammalian viral DNA polymerases3; (4) the molar base ratio of the high molecular weight RNA genome of RDV differs significantly from that of the FLV released from RD cells infected with FLV4. Thus, the suggestion was made that RDV might be a human type-C virus originating from the rhabdomyosarcoma cells of the RD cell line1.

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References

  1. McAllister, R. M., Nicolson, M., Gardner, M. B., Rongey, R. W., Rasheed, S., Sarma, P. S., Huebner, R. J., Hatanaka, M., Oroszlan, S., Gilden, R. V., Kabigting, A., and Vernon, L., Nature new Biol., 235, 3 (1972).

    Article  CAS  Google Scholar 

  2. Oroszlan, S., Bova, D., Martin-White, H. M., Toni, R., Foreman, C., and Gilden, R. V., Proc. natn. Acad. Sci., U.S.A., 59, 1211 (1972).

    Article  Google Scholar 

  3. Scolnick, E. M., Parks, W. P., Todaro, G. J., and Aaronson, S. A., Nature new Biol., 235, 35 (1972).

    Article  CAS  Google Scholar 

  4. Roy-Burman, P., and Kaplan, M. B., Biochem. biophys. Res. Commun., 48, 1354 (1972).

    Article  CAS  Google Scholar 

  5. Temin, H. M., Natn. Cancer Inst. Monog., 17, 557 (1964).

    Google Scholar 

  6. Neiman, P. E., Science, 178, 750 (1972).

    Article  CAS  Google Scholar 

  7. Neiman, P. E., Virology (in the press).

  8. Vogt, P. K., and Friis, R. R., Virology, 43, 223 (1971).

    Article  CAS  Google Scholar 

  9. Weiss, R. A., Friis, R. R., Katz, E., and Vogt, P. K., Virology, 46, 920 (1971).

    Article  CAS  Google Scholar 

  10. McAllister, R. M., Nelson-Rees, W. A., Johnson, E. Y., Rongey, R. W., and Gardner, M. B., J. Natn. Cancer Inst., 47, 603 (1971).

    CAS  Google Scholar 

  11. McAllister, R. M., Melnyk, J., Finklestein, J. Z., Adams, jun., E. C., and Gardner, M. B., Cancer, 24, 520 (1969).

    Article  CAS  Google Scholar 

  12. Robinson, W. S., Pitkanen, A., and Rubin, H., Proc. natn. Acad. Sci., U.S.A., 54, 137 (1965).

    Article  CAS  Google Scholar 

  13. Neiman, P. E., and Henry, P. H., Biochemistry, 10, 1733 (1971).

    Article  CAS  Google Scholar 

  14. Erikson, E., and Erikson, R. L., J. Virol., 8, 254 (1972).

    Google Scholar 

  15. McConaughy, B. L., and McCarthy, B. J., Biochim. biophys. Acta, 149, 18 (1967).

    Article  Google Scholar 

  16. Britten, R. J., and Kohne, D. E., Science, 161, 529 (1968).

    Article  CAS  Google Scholar 

  17. Gelderman, A. H., Rake, A. V., and Britten, R. J., Proc. natn. Acad. Sci., U.S.A., 68, 172 (1971).

    Article  CAS  Google Scholar 

  18. Melli, M., Whitfield, C., Rao, K. V., Richardson, M., and Bishop, J. O., Nature, 231, 8 (1971).

    CAS  Google Scholar 

  19. Grouse, L., Chilton, M., and McCarthy, B. J., Biochemistry, 11, 798 (1972).

    Article  CAS  Google Scholar 

  20. Straus, N. A., and Bonner, T. I., Biochim. biophys. Acta, 277, 87 (1972).

    Article  CAS  Google Scholar 

  21. Livingston, D. M., and Todaro, G. J., Virology (in the press).

Download references

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Authors and Affiliations

  1. Department of Medicine, University of Washington School of Medicine, Seattle, Washington, 98195

    PAUL E. NEIMAN

  2. US Public Health Service Hospital, Seattle, Washington, 98114

    PAUL E. NEIMAN

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  1. PAUL E. NEIMAN
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NEIMAN, P. Measurement of RD114 Virus Nucleotide Sequences in Feline Cellular DNA. Nature New Biology 244, 62–64 (1973). https://doi.org/10.1038/newbio244062a0

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