Supplementary Figure 9: Coexpression of ZnT8 variants.
From: Loss-of-function mutations in SLC30A8 protect against type 2 diabetes
HeLa cells overexpressed either (a) singly transfected C-terminal, V5-tagged ZnT8 variants or (b) cotransfections that also included C-terminally HA-tagged Trp325-ZnT8 for 24h, after which ZnT8 protein levels were observed. Cells were immunostained for ZnT8 expression using anti-HA and anti-V5 antibodies and costained with Hoechst 33342 to mark nuclei. Scale bars, 100μm (c) Protein blot analysis of HeLa cell lysates following cotransfection of Trp325-HA ZnT8 and V5-tagged ZnT8 variants. ZnT8 expression was detected using anti-V5 and anti-HA antibodies.
