Supplementary Figure 10: Inhibition of protein degradation.
From: Loss-of-function mutations in SLC30A8 protect against type 2 diabetes
HeLa cells transiently overexpressing V5-tagged ZnT8 variants were treated (+) with chloroquine (100 μM) or MG132 (10 μM) or left untreated in medium absent these chemicals, over a 4-h incubation period, to inhibit lysosomal and proteasomal degradation, respectively. Protein blot analysis was performed using anti-V5 and anti-tubulin antibodies.
