Supplementary Figure 3: Sanger sequencing traces of RHOA G17V mutation in T cell lymphoma patients.
From: A recurrent inactivating mutation in RHOA GTPase in angioimmunoblastic T cell lymphoma
Patient ID with RHOA G17V mutation is indicated by red text and arrows. Representative traces of wild-type and G17V mutant RHOA are shown in the top left panel. The horizontal red arrow shows the direction of genomic DNA sequencing. The mutant sequence shows a clear heterozygous mutation. Traces from tumor samples obtained from 45 AITL, 20 NK/T cell and 13 PTCL-NOS patients are shown in the rectangles. Patients 5, 6, 9, 10, 29, 30 and 43 in the AITL group showed the mutant as the dominant peak. The other 17 AITL patients showed the heterozygous mutation clearly. All three NK/T cell patients with somatic mutation showed the heterozygous genotype. Only one PTCL-NOS patient showed a heterozygous mutation. Sequencing trace chromatograms are representative of at least three independent experiments.
