Supplementary Figure 2: Elimination of the temperature-sensitive genome after multiple generations of selection at 29 °C.
From: Transmission of mitochondrial mutations and action of purifying selection in Drosophila melanogaster
The abundance of the temperature-sensitive mutant was measured by PCR amplifying a mtDNA region (mt1579–2799) using mtDNA from 30 adults as template followed by restriction digestion using XhoI in 4 heteroplasmic lines. (A) The upper panel shows that the mt:ND2del1 + mt:CoIT300I genome, when coexisting with wild type, declined to a few percent after ten generations of selection. The lower panel is Southern blot analysis of the digested PCR products, which showed disappearance of the temperature-sensitive allele in the tested heteroplasmic lines at generation 18. (B) mt:ND2del1 + mt:CoIT300I double-mutant genome was eliminated when coexisting with mt:ND2del1 in 2 of the 4 lines after 18 generations of selection.
