Supplementary Figure 9: Mito-GFP labels mitochondria. | Nature Genetics

Supplementary Figure 9: Mito-GFP labels mitochondria.

From: Selective propagation of functional mitochondrial DNA during oogenesis restricts the transmission of a deleterious mitochondrial variant

Supplementary Figure 9

(a) An ovariole expressing GFP fusion protein containing the mitochondrial-targeting sequence of citrate synthase (Mito-GFP)11 in germline cells, stained with an antibody against the ATP synthase α subunit (ATP-S; red) that labels mitochondria in both germ cells (arrowheads) and surrounding somatic cells (arrows). A representative image from >10 germaria is shown. Scale bar, 10 μm. (b) Enlarged view of the boxed region in a showing Mito-GFP and ATP-S colocalized at the fusome in region 2B of the germarium. Genotype: w1118/+;; nanos-Gal4, UAS-Mito-GFP/+. Scale bar, 10 μm.

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