Supplementary Figure 12: Quantification of heteroplasmy. | Nature Genetics

Supplementary Figure 12: Quantification of heteroplasmy.

From: Selective propagation of functional mitochondrial DNA during oogenesis restricts the transmission of a deleterious mitochondrial variant

Supplementary Figure 12

(a) Quantification of wild-type mtDNA product amplified by PCR from mixed template (wild type and mt:CoIT300I) and digested with XhoI compared to the amount of wild-type DNA in a digest containing a mix of wild-type and mt:CoIT300I PCR products. Quantified levels were plotted against the actual levels in the mix, and overlap of the two standard curves indicates a lack of impact of the wild type/mt:CoIT300I heteroduplex during PCR on quantification. (b) Quantification of the mt:CoIT300I level throughout fly development. E, embryo; 1L, first instar larvae; 2L, second instar larvae; 3L, third instar larvae; EP, early pupae; LP, late pupae; A, adult. Value, mean ± s.d of 3 biological replicates, each containing 50 flies.

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