Supplementary Figure 1: mtDNA replication in the Drosophila germarium visualized by EdU incorporation. | Nature Genetics

Supplementary Figure 1: mtDNA replication in the Drosophila germarium visualized by EdU incorporation.

From: Selective propagation of functional mitochondrial DNA during oogenesis restricts the transmission of a deleterious mitochondrial variant

Supplementary Figure 1

(a) Representative confocal section of a wild-type germarium showing EdU incorporation (green), mitochondria marked by α-ATP synthase α subunit staining (red) and nuclei labeled with DAPI staining (blue) in the absence of the DNA polymerase-α inhibitor aphidicolin or (b) with preincubation with aphidicolin. Without aphidicolin, intense EdU signals label nuclei (arrows) (a). In the presence of aphidicolin, nuclear incorporation is reduced and many puncta were localized within mitochondria (arrowheads) (b). Scale bars, 10 μm.

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