Supplementary Figure 5: Impaired respiration of mt:CoIT300I mitochondria and reduced activity of mt:CoIT300I COX at the non-permissive temperature.
(a) Average RCRs of three biological replicates of wild-type and mutant mitochondria purified from flies that eclosed at 18 °C and were cultured at 29 °C for 0, 2 or 4 d. Value, mean ± s.d. Mutant mitochondria have significant reduction in the RCR compared to the wild type after shifting to 29 °C, *P < 0.01, **P < 0.001. (b) COX activities of extracts from wild-type and mt:CoIT300I flies that eclosed and were maintained at 18 °C for 1 d. Extracts were incubated at 29 °C or 18 °C for 40 min, and COX activity was examined every 10 min. Data represent three biological replicates. Values were normalized with the average COX activity of wild type at 18 °C and 0 min, mean ± s.d.
