Supplementary Figure 6: Analysis from two cases that showed abnormal FISH results. | Nature Genetics

Supplementary Figure 6: Analysis from two cases that showed abnormal FISH results.

From: Germline and somatic SMARCA4 mutations characterize small cell carcinoma of the ovary, hypercalcemic type

Supplementary Figure 6

(a) Hematoxylin and eosin staining of UN11 and UN10. (b) SMARCA4 staining. (c) Interphase FISH analysis showing homozygous deletion of the SMARCA4 locus in cases UN11 and UN10 evaluated using Zeiss fluorescence microscopes equipped with appropriate filter sets and documented using ISIS software (MetaSystems, Altlusheim). The left panel for each case shows hybridization with the double-color (red and green) break-apart probe for SMARCA4, and the right panel shows hybridization of the same area with the control probe for the centromere of chromosome 6 (CEP6 labeled in blue, false color display as green to contrast nuclear counterstain in DAPI). Both cases show a significant number of cells lacking SMARCA4 signals but clearly showing CEP6 signals indicating homozygous loss. (d) Chromatograms of the mutations found in both cases.

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