Supplementary Figure 5: 35S gel showing the in vitro translation products of wild-type POT1 and OB domain mutants.
From: POT1 loss-of-function variants predispose to familial melanoma
This gel confirms that each in vitro translation reaction successfully produced protein for the electrophoretic mobility shift assay shown in Figure 2b. The p.Tyr89Cys, p.Gln94Glu and p.Arg273Leu POT1 variants were identified by exome sequencing familial melanoma cases. The p.Tyr223Cys variant was somatically acquired in CLL and has previously been shown to be unable to bind to telomeric DNA2. The DNA-protein complexes shown in Figure 2b were visualized by 32P labeling of (TTAGGG)3 single-stranded DNA (Online Methods).
